determination of herpetic keratitis frequency using loop mediated isothermal amplification (lamp) method in isfahan, iran

نویسندگان

maryam tarighati

vajihe karbasizade

sharareh moghim

چکیده

herpetic keratitis which is resulted from herpes simplex virus type i, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. the best method to diagnose it is molecular method which has high sensitivity and exclusivity. the current study was done on 25 eye samples collected from patients referred to the feyz hospital of isfahan who were diagnosed with herpetic keratitis disease. after cornea sampling and extracting dna from eye samples, lamp-pcr technique was done to search genome of herpes simplex virus type i. among 25 suspected cases of herpetic keratitis, 5 samples were confirmed to have genome virus of hsv1 and herpetic keratitis frequency was determined by 20%. the results of this investigation showed a relative high frequency of herpetic keratitis in this zone using lamp-pcr diagnostic method compared with similar studies which indicates high sensitivity of this technique. therefore, using such rapid, accurate and cheap molecular method is suggested

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Determination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran

Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

متن کامل

Determination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran

Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

متن کامل

Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification

We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole ...

متن کامل

LOOP mediated isothermal AMPlification (LAMP) in diagnosis of neorocryptococcosis

As a result of the importance of quick diagnosis of Neurocryptococcosis in patients, generally, molecular techniques are preferred rather than traditional methods for their rapidity. In this research, we made an effort to design and optimize Loop mediated isothermal AMPlification (LAMP) technique using specific primers for ura5 as the target gene for detection of Cryptococcus neoformans and Cry...

متن کامل

Malaria Diagnosis by Loop-mediated Isothermal Amplification (lamp) in Thailand.

The loop-mediated isothermal amplification method (LAMP) is a recently developed molecular technique that amplifies nucleic acid under isothermal conditions. For malaria diagnosis, 150 blood samples from consecutive febrile malaria patients, and healthy subjects were screened in Thailand. Each sample was diagnosed by LAMP, microscopy and nested polymerase chain reaction (nPCR), using nPCR as th...

متن کامل

تشخیص بیماریها با استفاده از (Loop-Mediated Isothermal Amplification (LAMP در دامپزشکی

 مقدمه و هدف: نیاز به روشی ساده تر، با کارایی بالاتر، سریعتر و ارزانتر نسبت به روشهای موجود جهت تشخیص بیماریهای دامپزشکی روش واکنش زنجیره ای پلی مراز (PCR) ، روشی سریع و حساس می باشد که قادر است تعداد نسخه اندکی از DNA هدف را تا سطحی که توسط ژل الکترفورز قابل تشخیص باشد، تکثیر نماید.بعلت کارایی بالا و قدرت زیاد PCR ، این روش در سالهای اخیر بطور گسترده ای در تشخیص پاتوژن های مختلف بیماریزا اعم ا...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of advanced biological and biomedical research

ناشر: casrp publishing company

ISSN 2383-2762

دوره 1

شماره 11 2013

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023